Laminin Solution

Laminin (Sigma # L 2020, 1mg/ml) Aliquot into 20μl units Store at -20ºC • Add diluted Laminin solution to cell suspension at a ratio of 1 part Laminin solution to 3 parts cell suspension.

Sodium borate solution (0.19M)

7.6 g Na2B4O7 (Sigma # 221732) 200ml DDW pH 8.2

Poly- D- lysine solution (1mg/ml)

10mg Poly-D -lysine (Sigma# P 1024) 10ml Sodium borate solution Filter Sodium borate solution only Keep for 30 minutes at room temperature, Store at -4º

Borate Buffer pH 8.5

2.375g Na2B4O7*10 H2O (Sodium Tetraborate) (Sigma # S 9640) 1.550g H3BO3 (Boric Acid) (Sigma # B 6768) 500ml DDW pH-8.5 with 1M HCL
Filter and store at 4◦C

PolyEthylenImine (PEI) solution (100μg/ml)

0.1ml or 0.107g 50% PEI (Sigma# P 3143, 50ml)
49.9ml Borate Buffer Aliquot into 1ml amounts, Store at -70◦C Before using : thaw 1ml aliquots and add 9ml Borate Buffer Store rest at -20◦ C for no more than 1 mounth.

Coating coverslips

Use about 50μl/cm2 Poly-D-lysine / Poly-Ethylene-Immine solution Coat overnight at room temperature, Aspirate solution using sterile Pasteur pipette
Wash in DDW, Dry with sterile Pasteur pipettes.